| 复合诱变选育衣康酸高产菌株的研究 |
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| 引用本文： | 杨静,蒋剑春,张飞,张宁,卫民,赵剑.复合诱变选育衣康酸高产菌株的研究[J].生物质化学工程,2015,49(3):13-16. |
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| 作者姓名： | 杨静 蒋剑春 张飞 张宁 卫民 赵剑 |
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| 作者单位： | 1. 中国林业科学研究院 林产化学工业研究所; 生物质化学利用国家工程实验室; 国家林业局 林产化学工程重点开放性实验室; 江苏省 生物质能源与材料重点实验室, 江苏 南京 210042;
2. 江苏警官学院 刑事科学技术系, 江苏 南京 210031 |
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| 基金项目： | 中国林科院中央级公益性科研院所基本科研业务费专项资金，“十二五”国家科技支撑计划资助 |
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| 摘 要： | 采用紫外线-LiCl、硫酸二乙酯(DES)复合诱变,经平板菌落筛选、摇瓶筛选以及连续传代稳定性考察,从土曲霉(Aspergillus terreus)2433出发菌株获得一株衣康酸高产突变株土曲霉At DES-11,其产酸量达到48.22 g/L,较出发菌株提高了156.08%;糖酸转化率为61.82%,较出发菌株提高了101.36%,且连续传代5次后,遗传性状仍较稳定,产酸量稳定在48 g/L左右,菌落及菌丝形态基本不变。
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| 关 键 词： | 土曲霉 衣康酸 复合诱变 |
| 收稿时间： | 2014-12-04 |
Compound Mutation Breeding of Strain with High Itaconic Acid Yield |
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| YANG Jing,JIANG Jian-chun,ZHANG Fei,ZHANG Ning,WEI Min,ZHAO Jian.Compound Mutation Breeding of Strain with High Itaconic Acid Yield[J].Biomass Chemical Engineering,2015,49(3):13-16. |
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| Authors: | YANG Jing JIANG Jian-chun ZHANG Fei ZHANG Ning WEI Min ZHAO Jian |
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| Affiliation: | 1. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab.for Biomass Chemical Utilization; Key and Open Lab.of Forest Chemical Engineering, SFA; Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
2. Criminal Science and Technology Department, Jiangsu Police Institute, Nanjing 210031, China |
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| Abstract: | By the compound mutation of UV-LiCl and diethyl sulfate (DES), flat screening of colony, shaker screening, and stability test of continuous passage, a mutant strain of Aspergillus terreus At DES-11 with high yield of itaconic acid was obtained from A.terreus2433.Its itaconic acid productivity and conversion rate of glucose were 48.22 g/L and 61.82% with an increase of 156.08% and 101.36% compared with those of original strain, respectively.And the heredity characteristics of the mutant strain were stable after cultivation for five continuous generations. |
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| Keywords: | Aspergillus terreus itaconicacid compound mutation |
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