Mutation effect of MeV protons on bioflocculant bacteria Bacillus cereus |
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| Authors: | Y.N. Yang N. Ren J. Yang |
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| Affiliation: | a Department of Environmental Engineering, Beihang University, 37th Xueyuan Road, Haidian District, P.O. Box 106, 100083 Beijing, PR China
b Institute of heavy Ion Physics of Peking University, 100083 Beijing, PR China |
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| Abstract: | A 3.2 MeV proton beam was used to irradiate bioflocculant bacteria (Bacillus cereus) to achieve mutation. The ion fluence ranged from 1011 to 1014/cm2. Most of the bacteria were killed when the ion fluence reached 1012 ions/cm2. The survival ratio drops in an exponential way on further increasing the ion fluence. The flocculating activity of 7 samples out of 51 showed a positive change, and a perfect mutant C7-23 with a stable high capacity of bioflocculant production was found. RAPD measurements showed that a new lane appears in this sample. The flocculating activity of the C7-23 bacteria increased by factors of 22%, 54% and 217% under pH values of 4, 7 or 10, respectively. |
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| Keywords: | 47.54.Fj 82.80.&minus d |
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