| MTS染色法测定重组人肿瘤坏死因子相关凋亡诱导配体的生物学活性 |
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| 引用本文： | 陈红霞,于玉根,张慧娟,黄立勤. MTS染色法测定重组人肿瘤坏死因子相关凋亡诱导配体的生物学活性[J]. 中国生物制品学杂志, 2011, 24(1) |
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| 作者姓名： | 陈红霞 于玉根 张慧娟 黄立勤 |
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| 作者单位： | 深圳新鹏生物工程有限公司; |
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| 摘 要： | 目的 建立稳定可靠的重组人肿瘤坏死因子相关凋亡诱导配体(Recombinant human TNF-related apoptosis-induc-ing ligand,rhTRAIL)生物学活性检测方法——MTS染色法。方法采用人非小细胞肺癌细胞(NCI-H460)凋亡-MTS染色法对3批rhTRAIL供试品进行检测,采用四参数回归法计算供试品效价;并对该方法的专属性、精密性和准确性进行验证。结果 3批供试品(20071101、20071102、20071103)的效价分别为7.3×104、8.1×104和9.3×104 U/ml,相关系数(R2)分别为0.998、0.996和0.998。rhTRAIL抗体能完全阻断rhTRAIL与NCI-H460细胞间的应答反应。该方法专属性较好,不同检测板间、不同加样位置、不同工作日间和不同试验人员间检测结果的平均变异系数分别为12%、26%、24%和7%,平均加样回收率为114%。结论 MTS染色法测定rhTRAIL的生物学活性具有较高的准确性和可信度,适用于rhTRAIL生物学活性的常规检测。
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| 关 键 词： | TNF相关凋亡诱导配体 生物学活性 MTS |
Determination of Bioactivity of Recombinant Human TNF-related Apoptosis-inducing Ligand by MTS Staining |
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| CHEN Hong-xia,YU Yu-gen,ZHANG Hui-juan,HUANG Li-qin. Determination of Bioactivity of Recombinant Human TNF-related Apoptosis-inducing Ligand by MTS Staining[J]. Chinese Journal of Bilogicals, 2011, 24(1) |
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| Authors: | CHEN Hong-xia YU Yu-gen ZHANG Hui-juan HUANG Li-qin |
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| Affiliation: | CHEN Hong-xia,YU Yu-gen,ZHANG Hui-juan,HUANG Li-qin(Shenzhen Xinpeng Bio-tech Co.Ltd,Shen-zhen 518057,Guangdong Province,China) |
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| Abstract: | Objective To develop a stable and reliable MTS staining method for determination of bioactivity of recombinant human TNF-related apoptosis-inducing ligand(rhTRAIL).Methods Three batches of test samples of rhTRAIL were determined by induction of human non-small cell lung cancer NCI-H460 cell apoptosis and MTS staining,and calculated for potency by four-parame-ter regression method,based on which the specificity,precision and accuracy of the developed method were verified.Results The potencies of the three ba... |
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| Keywords: | TNF-related apoptosis-inducing ligand(TRAIL) Bioactivity MTS |
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