| RV-G/LTB双基因融合真核表达质粒的构建及其在Vero细胞中的表达 |
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| 引用本文： | 刘娟,刘明远,于录,郭恒,李扬,赵丽晶,孙树民,李慧萍,刘学,王学林.RV-G/LTB双基因融合真核表达质粒的构建及其在Vero细胞中的表达[J].粉末涂料与涂装,2011,24(3):255-258. |
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| 作者姓名： | 刘娟 刘明远 于录 郭恒 李扬 赵丽晶 孙树民 李慧萍 刘学 王学林 |
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| 作者单位： | 人兽共患病研究教育部重点实验室吉林大学人兽共患病研究所; |
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| 摘 要： | 目的 构建RV-G/LTB双基因融合真核表达质粒,并在Vero细胞中表达.方法 应用重叠延伸PCR方法,采用一定的linker序列(Gly4Ser)2将已克隆的狂犬病病毒糖蛋白(Rabies virus glycoprotein,RV-G)基因片段和大肠杆菌不耐热肠毒素B亚单位(E.coli heat-labile e...
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| 关 键 词： | 狂犬病病毒 糖蛋白类 大肠杆菌不耐热肠毒素B 融合基因 Vero细胞 |
Construction of Eukaryotic Expression Vector for Fusion Gene RV-G/LTB and Its Expression in Vero Cells |
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| LIU Juan,LIU Ming-yuan,YU Lu,GUO Heng,LI Yang,ZHAO Li-jing,SUN Shu-min,LI Hui-ping,LIU Xue,WANG Xue-lin.Construction of Eukaryotic Expression Vector for Fusion Gene RV-G/LTB and Its Expression in Vero Cells[J].Chinese Journal of Biologicals,2011,24(3):255-258. |
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| Authors: | LIU Juan LIU Ming-yuan YU Lu GUO Heng LI Yang ZHAO Li-jing SUN Shu-min LI Hui-ping LIU Xue WANG Xue-lin |
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| Affiliation: | LIU Juan,LIU Ming-yuan,YU Lu,GUO Heng,LI Yang,ZHAO Li-jing,SUN Shu-min,LI Hui-ping,LIU Xue,WANG Xue-lin(Key Laboratory of Zoonosis Research,Ministry of Education,Institute of Zoonosis,Jilin University,Changchun 130062,China) |
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| Abstract: | Objective To construct a eukaryotic expression vector for fusion gene RV-G / LTB and express in Vero cells.Methods The cloned gene fragments encoding rabies virus(RV) glycoprotein(G) and E.coli heat-labile enterotoxin subunit B(LTB) were fused by SOE-PCR using a linker sequence(Gly4Ser)2 and inserted into eukaryotic expression vector pVAX1.The constructed recombinant plasmid pVAX1-G-linker-LTB was transfected to Vero cells,and the expressed fusion protein was identified by indirect IFA and Western blot.Resu... |
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| Keywords: | Rabies virus Glycoproteins E coli heat-labile enterotoxin subunit B(LTB) Fusion gene Vero cells |
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