| 嗜水气单胞菌J-1株灭活疫苗生产工艺的改进 |
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| 引用本文： | 陶家发,赖迎迢,任燕,江小燕,康光辉,张悠,石存斌,吴淑勤. 嗜水气单胞菌J-1株灭活疫苗生产工艺的改进[J]. 中国生物制品学杂志, 2011, 24(3): 345-348 |
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| 作者姓名： | 陶家发 赖迎迢 任燕 江小燕 康光辉 张悠 石存斌 吴淑勤 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所广东省水产动物免疫技术重点实验室; |
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| 基金项目： | 农业公益性行业科研专项(200803013); 农业科技成果转化资金项目(2008GB23260393) |
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| 摘 要： | 目的 改进嗜水气单胞菌J-1株灭活疫苗的生产工艺,提高培养效率.方法 采用改良培养基和产毒素培养基,利用机械式通风搅拌培养J-1菌株.确定发酵培养时间,并比较两种培养基的培养效果;用摇瓶灭活和罐灭活J-1菌株培养液,比较不同浓度甲醛的灭活效果.结果 用机械搅拌发酵罐培养J-1菌株,菌体生长12～14 h达峰值,产毒素培...
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| 关 键 词： | 嗜水气单胞菌 疫苗,灭活 生产工艺 |
Improvement of Production Procedure for Inactivated Vaccine against Aeromonas hydrophila J-1 Strain |
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| TAO Jia-fa,LAI Ying-tiao,REN Yan,JIANG Xiao-yan,KANG Guang-hui,ZHANG You,SHI Cun-bin,WU Shu-qin. Improvement of Production Procedure for Inactivated Vaccine against Aeromonas hydrophila J-1 Strain[J]. Chinese Journal of Bilogicals, 2011, 24(3): 345-348 |
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| Authors: | TAO Jia-fa LAI Ying-tiao REN Yan JIANG Xiao-yan KANG Guang-hui ZHANG You SHI Cun-bin WU Shu-qin |
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| Affiliation: | TAO Jia-fa,LAI Ying-tiao,REN Yan,JIANG Xiao-yan,KANG Guang-hui,ZHANG You,SHI Cun-bin,WU Shu-qin(Pearl River Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Guangdong Key Laboratory of Aquatic Animal Immune Techniques,Guangzhou 510380,China) |
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| Abstract: | Objective To improve the production procedure for inactivated vaccine against Aeromonas hydrophila J-1 strain and increase the culture efficiency.Methods J-1 strain was cultured in modified and toxin-producing media by mechanical stirring under ventilating condition,based on which the time for fermentation was determined,and the culture effets of the two media were compared.The culture liquid of J-1 strain was inactivated in shake-flask and in inactivation tank respectively,and the inactivation effects by f... |
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| Keywords: | Aeromonas hydrophila Vaccine inactivated Production procedure |
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