吲哚美辛对人羊膜上皮细胞水通道蛋白8表达的影响

目的 探讨吲哚美辛对人羊膜上皮细胞水通道蛋白(Aquaporin,AQP)8表达的影响.方法 原代培养人羊膜上皮细胞,采用免疫细胞化学法进行鉴定.将细胞分为实验组和对照组,实验组以不同浓度的吲哚美辛(10、20、50、100、200 μmol/L)作用24 h,另以200 μmol/L吲哚美辛分别作用6、12、24、3...

Effect of Indomethacin on Expression of Aquaporin 8 in Human Amniotic Epithelial Cells

Objective To investigate the effect of indomethacin on expression of aquaporin 8(AQP 8) in human amniotic epithelial cells.Methods The primary culture of human amniotic epithelial cells were identified by immuocytochemistry,then treated with indomethacin at various concentrations(10,20,50,100 and 200 μmol / L)for 24 h respectively,using the cells in normal culture as control.On the basis of this,another group of human amniotic membrane cells were treated with 200 μmol / L indomethacin for 6,12,24,36 and 48 h respectively.The expressions of AQP 8 mRNA and protein in amniotic membrane cells of various groups were determined by RT-PCR and Western blot respectively.Results Except those at a concentration of 10 μmol / L,the indomethacin at other concentrations down-regulated the expressions of AQP 8 mRNA and protein significantly(P < 0.01),while the expression levels in the cells treated with 200 μmol / L indomethacin was the lowest.The expression levels of AQP 8 mRNA and protein in the cells treated with 200 μmol / L indomethacin for more than 12 h were significantly lower than those in control groups(P <0.01),which decreased in a time-dependent mode and reached the minimum 48 h after treatment.Conclusion Indomethacin down-regulated the expression of AQP 8 mRNA and protein in human amniotic epithelial cells.