Effect of dietary lipids on the lipid composition and phospholipid deacylating enzyme activities of rat heart |
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Authors: | Jeannie Leonardi Elise Termine Florence Morand Raymond Lafont Henri Portugal Huguette Lafont Gilles Nalbone |
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Affiliation: | (1) Laboratoire de Geologie du Quaternaire, CNRS, Marseille, France;(2) Laboratoire Central (Professeur A.M. Pauli), Hopital Ste-Marguerite, Marseille, France;(3) INSERM U-130, 10 avenue Viton, 13009 Marseille, France |
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Abstract: | Rats were fed lard-enriched (17%) or corn oil-enriched (17%) diets and were compared with rats fed a low fat (4.5%) diet.
Cardiac protein, DNA, phospholipid (PL) and fatty acid (FA) compositions were analyzed. Neutral phospholipase A, lysophospholipase
and creatine kinase activities in the membrane and cytosolic compartments were also investigated.
No significant modification of cardiac protein, DNA nor PL was observed among the three groups. Some alterations appeared
in the FA composition. A lard-enriched diet induced a significant increase of 22∶5n−3 and 22∶6n−3 in heart phosphatidylcholine
(PC) and phosphatidylethanolamine (PE), whereas a linoleic acid-rich diet induced a specific increase of 22∶4n−6 and 22∶5n−6
in these two major PL.
Compared to rats fed the low fat diet, membrane-associated phospholipase A activity, measured by endogenous hydrolysis of
membrane PC and PE, showed a significant increase (+45%) for both PL in rats fed corn oil. However, the activity of membrane-associated
phospholipases, measured with exogenous 1-14C]dioleoyl PC, was not different among the three groups of rats. Cytoplasmic activity was decreased in rats fed corn oil,
and lysophospholipase and creatine phosphate kinase activities were not significantly affected by diet.
FA modification of the long chain n−6 FA induced by corn oil may be responsible for the observed increase in phospholipase
activity. Physiological implications are suggested in terms of membrane degradation and prostaglandin production.
Presented in part at the International Symposium on Lipid Metabolism in the Normoxic and Ischemic Heart, Rotterdam, The Netherlands,
September 1986. |
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