Homoserine Lactones,Methyl Oligohydroxybutyrates,and Other Extracellular Metabolites of Macroalgae‐Associated Bacteria of the Roseobacter Clade: Identification and Functions |
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| Authors: | Lisa Ziesche Hilke Bruns Marco Dogs Laura Wolter Florian Mann Prof Irene Wagner‐Döbler Prof?Dr Thorsten Brinkhoff Prof Stefan Schulz |
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| Affiliation: | 1. Institute of Organic Chemistry, Technische Universit?t Braunschweig, Braunschweig, Germany;2. Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Oldenburg, Germany;3. Helmholtz Centre for Infection Research, Braunschweig, Germany |
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| Abstract: | Twenty‐four strains of marine Roseobacter clade bacteria were isolated from macroalgae and investigated for the production of quorum‐sensing autoinducers, N‐acylhomoserine lactones (AHLs). GC/MS analysis of the extracellular metabolites allowed us to evaluate the release of other small molecules as well. Nineteen strains produced AHLs, ranging from 3‐OH‐C10:0‐HSL (homoserine lactone) to (2E,11Z)‐C18:2‐HSL, but no specific phylogenetic or ecological pattern of individual AHL occurrence was observed when cluster analysis was performed. Other identified compounds included indole, tropone, methyl esters of oligomers of 3‐hydroxybutyric acid, and various amides, such as N‐9‐hexadecenoylalanine methyl ester (9‐C16:1‐NAME), a structural analogue of AHLs. Several compounds were tested for their antibacterial and antialgal activity on marine isolates likely to occur in the habitat of the macroalgae. Both AHLs and 9‐C16:1‐NAME showed high antialgal activity against Skeletonema costatum, whereas their antibacterial activity was low. |
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| Keywords: | quorum sensing antialgal activity autoinducer macroalgae polyhydroxybutyrate |
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