In Vitro Reconstitution of Formylglycine‐Generating Enzymes Requires Copper(I) |
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Authors: | Matthias Knop Pascal Engi Roxana Lemnaru Prof Dr Florian P Seebeck |
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Affiliation: | Department of Chemistry, University of Basel, Basel, Switzerland |
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Abstract: | Formylglycine‐generating enzymes (FGEs) catalyze O2‐dependent conversion of specific cysteine residues of arylsulfatases and alkaline phosphatases into formylglycine. The ability also to introduce unique aldehyde functions into recombinant proteins makes FGEs a powerful tool for protein engineering. One limitation of this technology is poor in vitro activity of reconstituted FGEs. Although FGEs have been characterized as cofactor‐free enzymes we report that the addition of one equivalent of CuI increases catalytic efficiency more than 20‐fold and enables the identification of stereoselective C?H bond cleavage at the substrate as the rate‐limiting step. These findings remove previous limitations of FGE‐based protein engineering and also pose new questions about the catalytic mechanism of this O2‐utilizing enzyme. |
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Keywords: | copper formylglycine kinetic isotope effects oxygen activation protein engineering |
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