GLUE That Sticks to HIV: A Helix‐Grafted GLUE Protein That Selectively Binds the HIV gp41 N‐Terminal Helical Region |
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| Authors: | Susanne N Walker Rachel L Tennyson Alex M Chapman Prof Alan J Kennan Prof Brian R McNaughton |
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| Affiliation: | 1. Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523 (USA);2. Department of Chemistry, Colorado State University, Fort Collins, CO 80523 (USA) |
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| Abstract: | Methods for the stabilization of well‐defined helical peptide drugs and basic research tools have received considerable attention in the last decade. Here, we report the stable and functional display of an HIV gp41 C‐peptide helix mimic on a G RAM‐L ike U biquitin‐binding in E AP45 (GLUE) protein. C‐peptide helix‐grafted GLUE selectively binds a mimic of the N‐terminal helical region of gp41, a well‐established HIV drug target, in a complex cellular environment. Additionally, the helix‐grafted GLUE is folded in solution, stable in human serum, and soluble in aqueous solutions, and thus overcomes challenges faced by a multitude of peptide drugs, including those derived from HIV gp41 C‐peptide. |
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| Keywords: | gp41 helix grafting HIV/AIDS protein engineering protein– protein interactions |
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