Structurally Diverse Polyamines: Solid‐Phase Synthesis and Interaction with DNA |
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| Authors: | Dr Naoki Umezawa Yuhei Horai Dr Yuki Imamura Makoto Kawakubo Mariko Nakahira Dr Nobuki Kato Akira Muramatsu Prof Dr Yuko Yoshikawa Prof Dr Kenichi Yoshikawa Prof Dr Tsunehiko Higuchi |
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| Affiliation: | 1. Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467‐8603 (Japan);2. Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, 610‐0394 (Japan);3. Research Organization of Science and Technology, Ritsumeikan University, Kusatsu 525‐8577 (Japan) |
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| Abstract: | A versatile solid‐phase approach based on peptide chemistry was used to construct four classes of structurally diverse polyamines with modified backbones: linear, partially constrained, branched, and cyclic. Their effects on DNA duplex stability and structure were examined. The polyamines showed distinct activities, thus highlighting the importance of polyamine backbone structure. Interestingly, the rank order of polyamine ability for DNA compaction was different to that for their effects on circular dichroism and melting temperature, thus indicating that these polyamines have distinct effects on secondary and higher‐order structures of DNA. |
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| Keywords: | amines DNA structures peptides single‐molecule studies solid‐phase synthesis |
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