CONCENTRATION OF GAMMA LINOLENIC ACID (GLA) FROM BORAGE OIL BY UREA COMPLEXATION: OPTIMIZATION OF REACTION CONDITIONS |
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| Authors: | SHARON A SPURVEY FEREIDOON SHAHIDI |
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| Affiliation: | Department of Biochemistry Memorial University of Newfoundland St. John's, Newfoundland, A1B 3X9, Canada |
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| Abstract: | Production of gamma-linolenic acid (GLA, 18:3ω6) concentrates from borage oil (BO) was optimized. A 3-factor-3-level face-centered cube design was used to study the effect of reaction time (X1), reaction temperature (X2) and urea-to-fatty acid ratio (X3) in order to maximize the GLA content(Y). A second-order polynomial model was employed to generate a surface response. Under optimum conditions, a maximum of 91% GLA was obtained from borage oil at a reaction time of 16 h, reaction temperature of -7C and a urea-to-fatty acid ratio(w/w) of 3.7. |
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