氟吡菌酰胺及其代谢物在烟草种植和加工过程中的降解特征

为探究氟吡菌酰胺及其代谢物AE C656948-Benzamide在烟草种植和加工过程中的残留降解特征，建立了QuEChERS-超高效液相色谱-串联质谱（QuEChERS-UPLC-MS/MS）法检测烟叶中氟吡菌酰胺及其代谢物残留量。结果表明，在0.05~10 mg/kg添加水平下，氟吡菌酰胺在鲜烟叶和干烟叶中的平均回收率分别为86%~94%和81%~95%，AE C656948-Benzamide在鲜烟叶和干烟叶中的平均回收率分别为85%~91%和83%~89%，相对标准偏差（RSD）均<10%，定量限（LOQ）均为0.05 mg/kg。氟吡菌酰胺在田间前3 d内消解率>50%，后期消解缓慢。烘烤过程中，氟吡菌酰胺的消解率为58.1%，AE C656948-Benzamide的消解率为52.9%，加工因子（Pf）为0.42~0.47。陈化360 d，氟吡菌酰胺的消解率为1.8%，AE C656948-Benzamide未发生降解，Pf为0.99~1.54。总之，烟叶中的氟吡菌酰胺和AE C656948-Benzamide在烟草种植和烘烤过程中降解明显，在陈化过程降解甚少。

Residue Degradation of Fluopyram and its Metabolites during Tobacco Planting and Processing

To investigate the residue degradation of fluopyram and its metabolite AE C656948-Benzamide during tobacco planting and processing, the QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry (QuEChERS-UPLC-MS/MS) method was adopted to detect the residues of fluopyram and its metabolite in tobacco leaves. At addition levels of 0.05-10 mg/kg, the results showed that the average recoveries of fluopyram in fresh leaves and cured leaves were 86%-94% and 81%-95%, AE C656948-Benzamide were 85%-91% and 83%-89%, with relative standard deviations (RSD) less than 10%. The quantitative limits for fluopyram and AE C656948-Benzamide in fresh leaves and cured leaves were both 0.05 mg/kg. The degradation rate of fluopyram was more than 50% in the first 3 days, but it was slow after then. During curing the degradation rate of fluopyram residue was 58.1% and the degradation rate of AE C656948-Benzamide residue was 52.9%. The processing factors (Pf) were in the range of 0.42-0.47. The degradation rate of fluopyram was 1.8% and AE C656948-benzamide was not degraded within 360 days during aging. The Pf varied in the range of 0.99-1.54. In conclusion, fluopyram and AE C656948 benzamide degrade significantly during tobacco planting and baking, but rarely during aging.