Image processing based on the combination of high-resolution electron microscopy and electron diffraction

A method of crystal structure determination by electron crystallographic image processing based on the combination of high-resolution electron microscopy (HREM) and electron diffraction is introduced. It consists of two stages: image deconvolution and resolution enhancement. In the first stage an image taken at an arbitrary defocus condition is transformed into the structure image with the resolution depending on the resolution of the electron microscope. In the second stage the image resolution is enhanced to the diffraction resolution limit by combining the electron diffraction data and using the phase extension technique so that in the final image most unoverlapped atoms can be resolved individually. The experimental diffraction intensities are corrected for approximating to square structure factors. The principle of the image processing and the procedure of diffraction intensity correction are briefly described and the results of applications are illustrated. Since the method is based on the weak phase object approximation (WPOA), the validity of WPOA is discussed by introducing an approximate image contrast theory named pseudo weak phase object approximation (PWPOA) to demonstrate the image contrast change with the crystal thickness for very thin crystals.