碱联合超高压预处理对笋壳酶解效率的影响

以竹笋壳（BS）为原料，采用单独超高压（UHP）、单独碱加热（AH）、超高压后碱加热（UHP+AT）和碱加热后超高压（AT+UHP）4种方法进行预处理，并对比分析了样品预处理前后的化学组成、扫描电镜（SEM）、晶体结构（XRD）、红外光谱（FTIR）、比表面积和孔结构及酶水解效率的变化规律。结果表明：与其他处理方法相比，AT+UHP在450MPa压强下处理效果最好。预处理后样品中木质素的脱除率为86.87%，微观表面结构松散和粗糙，结晶指数有所上升，比表面积和孔体积分别为2.590m²/g、0.010cm³/g，酶水解效率高达97.89%。UHP+AT与AT+UHP预处理效果差异不明显，其酶解效率达到96.94%。因此，碱联合超高压处理是生物燃料生产中生物质预处理的一种潜在选择。

Effects of alkaline combined with ultra-high pressure pretreatment on enzymatic hydrolysis efficiency of bamboo shell

Bamboo shell (BS) was pretreated by ultra-high pressure (UHP), alkaline-heating method (AH), UHP combined with AH (UHP+AH) and AH combined with UHP (AH+UHP). The chemical composition was determined. And scanning electron microscopy (SEM), crystal structure (XRD), infrared spectroscopy (FTIR), specific surface area and pore structure, and enzymatic hydrolysis were used to analyze the changes of samples before and after pretreatment. The results showed that AT+UHP pretreatment had the best effect under 450MPa pressure compared with other pretreatment methods. After pretreatment with AT+UHP at 450MPa, 86.87% of the lignin was removed, the surface was loose and coarse, and the crystallization index increased, the specific surface area and pore size were 2.590m²/g and 0.010cm³/g, respectively, while the enzymatic hydrolysis efficiency was 97.89%. There was no obvious difference between the pretreatment effects of UHP+AT and AT+UHP. The enzymatic hydrolysis efficiency of UHP+AT pretreatment sample was up to 96.94%. Therefore, alkaline combined with ultra-high pressure pretreatment can be a potential alternative for biomass pretreatment in biofuel production.