不同产地辣木叶HPLC指纹图谱研究

目的 高效液相色谱法(HPLC)建立辣木叶指纹图谱,为辣木叶的质量控制和评价提供参考.方法 选用Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),检测波长230 nm,流动相为甲醇-0.05%磷酸水溶液梯度洗脱,流速1.0 ml/min,柱温为35℃,进样量10μl;以没食子酸为参照峰,在相同的色谱条件下测定9批不同产地的辣木叶药材,用软件"中药色谱指纹图谱相似度评价系统(2012版)"对9批辣木叶进行相关分析.结果 建立了辣木叶的HPLC指纹图谱,9批辣木叶的相似度在0.63-0.87之间,指定了15个共有峰,2个已确认成分,分别为没食子酸和异槲皮素;以对照图谱为对照,各共有峰的相对保留时间RSD均小于2.0%,各共有峰的相对峰面积差别显著,表明这9批辣木叶原材化学成分种类差别较小,各成分含量差别较大.结论 此法具有良好的精密度、重复性、稳定性,可为辣木叶的质量评价与控制提供科学依据.

HPLC Fingerprint Analysis of Moringa Oleifera Leaves from Different Habitats

Objective To establish fingerprint of Moringa oleifera leaves by HPLC,in order to provide reference for quality control and evaluation.Methods HPLC was performed on Diamonsil C18(4.6 mm×250 mm,5μm)column,with methanol-0.05%phosphoric acid as the mobile phase for gradient elution.The detection wavelength was 230 nm,the flow rate was 1.0 ml/min,the column temperature was set at 35℃,and the sample amount was 10μl.With gallic acid as reference substance,the fingerprints of 9 batches of Moringa oleifera leaves were analyzed under the same chromatographic conditions.The Similarity Evaluation System for Chinese medicine Chromatographic Fingerprints(2012 Edition)was applied for the analysis of 9 batches of Moringa oleifera leaves.Results The HPLC fingerprint of Moringa oleifera leaves was established.The similarity of the fingerprints of 9 batches of Moringa oleifera leaves was between 0.63-0.87.15 common fingerprint peaks were calibrated and 2 components were identified,namely gallic acid and isoquercetin.Compared with control chart,the RSD of retention time of each common peak was less than 2.0%.The relative peak area of each common peak is significantly different,indicating that the chemical composition of the 9 batches of Moringa oleifera leaves was not so far different,and the content of each component was quite different.Conclusion With a good precision,repeatability and stability,the method will provide a scientific basis to evaluate and control the quality of Moringa oleifera leaves.