SPME-GC/MS法分析红花芒毛苣苔中的挥发油化学成分

分析红花芒毛苣苔中的挥发油化学成分。采用固相微萃取法从红花芒毛苣苔中提取挥发油化学成分,应用气相色谱-质谱联用技术对其进行鉴定,采用峰面积归一化法计算各组分的相对百分含量。色谱条件:HP-5弹性石英毛细管色谱柱(30 m×0.25 mm,0.25μm);升温程序:初始温度45°C,保持2 min,然后以10°C/min的速度升至250°C并保持30 min;进样口温度250°C,流速0.8 mL/min,不分流进样。分离得到63个组分,鉴定出其中58个成分,所鉴定的组分占总峰面积的98.06%。采用固相微萃取技术进行提取具有操作时间短,样品用量小,无需萃取溶剂等优点。该结果为了解红花芒毛苣苔中的挥发性成分及开发应用该植物资源提供了参考依据。

Analysis of Chemical Constituents of Volatile Oil in Aeschynanthus moningeriae(Merr.)Chun by SPME-GC/MS

The chemical constituents of volatile oil in Aeschynanthus moningeriae(Merr.) were analyzed and extracted by SPME.The components of the volatile oil were separated and identified by GC-MS.The relative content of each component was determined by area normalization method.The working conditions were: HP-5 elastic quartz capillary-tube chromatographic column(30 m×0.25 mm,0.25 μm);keeping the initial temperature at 45(°C) for 2 minutes,and then increasing the temperature to 250(°C) at a rate of 10(°C)/min and keeping it for 30 minutes. The temperature at the sample-feeding entrance was 250(°C),and the flowing rate was 0.8 mL/min without any divided sample-feeding.Sixty three kinds of components were separated.Among them,fifty eight components were identified,accounting about 98.06% of the total chemical constituents.The technique of solid-phase microextraction has the advantages of short extracting time,small sample amount and no need of extracting solvent.This experiment result enhances our understanding of chemical constituents of volatile oils in Aeschynanthus moningeriae(Merr.) and provides reference for the development and utilization of the natural medicinal resource.