| Abstract: | A study of the size exclusion chromatographic behavior of styrene–methacrylic acid and epoxyacrylic polymers has been made. These polymers exhibit strong “ion-exclusion” effects in DMF, similar to that found for water-soluble polyelectrolytes such as sodium polystyrene sulfonate. The addition of lithium bromide, at a concentration of 0.1M, overcomes ion exclusion on a set of silicabased, “deactivated” duPont SEC columns. Calibration of both the duPont columns and a set of styrene–divinyl benzene based columns (μ-Styragel) is complicated by absorption of polystyrene standards in DMF and DMF/salt mobile phases. The absorption of monomeric solutes on the duPont columns in DMF/salt mobile phases is different from that of their corresponding polymers. |
