An interference technique for measuring the thickness of semi-thin and thick sections |
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Authors: | W. M. Robertson B. Storey B. S. Griffiths |
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Abstract: | An interference method is described for measuring section thickness in the range 0·3–45 μm. An incident illumination objective incorporating a beam splitter and adjustable reference mirror is used to generate interference fringes by reflection from the upper surfaces of sections on glass slides. Sections do not require a reflective coating. The lateral displacement of the zero-order fringe generated using white light is measured in terms of sodium light fringes and photographic enlargement of the fringes allows measurement to ± 30 nm. The method is simple in operation and allows rapid assessment of any local distortions over the entire section area. |
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Keywords: | Section thickness interference stereology microdensitometry |
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