Large-scale purification and characterisation of pea globulins |
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Authors: | Jacques Gueguen Anh T Vu Franois Schaeffer |
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Affiliation: | Jacques Gueguen,Anh T. Vu,François Schaeffer |
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Abstract: | Pea globulins, vicilin and legumin were isolated by chromatography on DEAE Sepharose and Ultrogel ACA 34. The procedure was carried out on a preparative scale and used to purify about 5 g of each globulin for a separation cycle. The purity of the vicilin and legumin was verified by immunoelectrophoretic and ultracentrifuge analysis. |
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Keywords: | Pea globulin vicilin legumin preparative chromatography |
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