The effect of steroids and their solubilizing agents on mycelial growth ofPhytophthora cactorum |
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Authors: | W. David Nes Glenn W. Patterson George A. Bean |
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Affiliation: | (1) Department of Botany, University of Maryland, 20742 College Park, MD |
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Abstract: | Mycelia ofPhytophthora cactorum, a fungus unable to synthesize sterols, were incubated with a series of sterols and several common sterol solubilizing agents. Cholesterol, campesterol, and sitosterol promoted growth, while carpesterol, saringosterol, 20 α-hydroxy cholesterol, coprostanol, and 5α-cholestane inhibited growth. Cholestanol, epicholestanol, and 5α-cholestan-3-one had no significant effect on growth relative to the sterol-free control. All steroids were solubilized in ethanol for studying structure-activity correlations. The added steroids were reisolated from mycelia and their identity confirmed by gas liquid chromatography (GLC) or combined gas liquid chromatography-mass spectrometry (GLC-MS). Tween 80 promoted growth, Triton X-100 inhibited growth, and ethanol had no effect on growth relative to a control. Scientific Article No. A2441, Contribution No. 5469 of the Maryland Agricultural Experiment Station. |
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