烟草愈伤组织的诱导及细胞悬浮培养体系的建立

以鄂烟1号无菌苗的叶为材料进行愈伤组织的诱导,筛选出生长培养基的最佳激素配比,初步建立了烟草细胞悬浮培养体系。结果表明,愈伤组织诱导的最优基本培养基为MS培养基,以MS NAA1.0mg/L 6-BA0.5mg/L为最佳配方;愈伤组织最佳生长培养基为MS 2,4-D0.1mg/L NAA1.5mg/L 6-BA0.3mg/L,愈伤组织培养第6天进入对数生长期,第14天干重增至最初干重的12.536倍,并以此配方建立了烟草细胞悬浮培养体系。

Tobacco Callus Induction and Establishment of Cell Suspension Culture System

The callus induction was carried out with the leaves of aseptic seedling of tobacco E'yan 1,and the optimal hormone proportion in culture medium was screened out,and the system of tobacco cell suspension culture was established preliminarily.The results showed that MS was the optimal basic medium for callus induction,and the optimal formula was MS NAA 1.0 mg/L 6-BA 0.5 mg/L;while the optimal formula for culture medium of callus was MS 2,4-D 0.1 mg/L NAA 1.5 mg/L 6-BA 0.3 mg/L.On the 6th day of callus culturing,the logarithm growth period started;and on the 14th day,the dry weight increased to 12.536 times of its initial dry weight.The system of tobacco cell suspension culture was established based on this formula.