Mammary transfer and metabolism in the rat of halogenated fatty acids of halogenated olive oil |
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Authors: | H B S Conacher R K Chadha J F Lawrence S M Charbonneau F Bryce |
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Affiliation: | (1) Bureau of Chemical Safety, Food Directorate, Health Protection Branch, K1A 0L2 Ottawa, Ontario, Canada |
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Abstract: | To assess possible incorporation of halogenated fatty acids into the neonate via the milk, a 4-day study was carried out in
which lactating Wistar rats were orally dosed with either brominated olive oil (BOO) (0.6 g/kg body wt/day) or chlorinated
olive oil (COO) (0.4 g/kg body wt/day) for the first 4 days. On days 1–5 inclusive 2 pups per litter were sacrificed and the
stomach curd and livers analyzed for halogenated fatty acids by gas liquid chromatography (GLC). On day 5 all dams also were
sacrificed and their livers and adipose tissue similarly analyzed. With BOO, brominated fatty acids (bfa) accumulated in both
the milk lipids and neonate liver lipids, and appeared to plateau on day 4 at levels of 2% and 5% respectively. In contrast
to the BOO in which approximately 100% of the bfa was dibromostearis (DBS), the milk bfa comprised 79% (DBS), 9% dibromopalmitic
(DBP) and 12% dibromomyristic (DBM) acids, suggesting maternal metabolism to the shorter chain brominated acids. In the neonate
liver lipids the bfa composition was 47% (DBS), 12% (DBP) and 41% (DBM), suggesting either further metabolism in the neonate
and/or preferential accumulation of the shorter chain brominated acids. The analysis of maternal tissue indicated very low
bfa residues, contrary to previous studies in non-lactating rats. Similar results were obtained with COO. |
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