| 巯基自组装修饰恒电位共价键合法固定基因电化学传感器的制备与表征 |
| |
| 引用本文： | 李清军,焦奎,孙伟,胡轩. 巯基自组装修饰恒电位共价键合法固定基因电化学传感器的制备与表征[J]. 青岛科技大学学报(自然科学版), 2005, 26(3): 193-196 |
| |
| 作者姓名： | 李清军 焦奎 孙伟 胡轩 |
| |
| 作者单位： | 青岛科技大学,化学与分子工程学院,山东,青岛,266042;青岛科技大学,化学与分子工程学院,山东,青岛,266042;青岛科技大学,化学与分子工程学院,山东,青岛,266042;青岛科技大学,化学与分子工程学院,山东,青岛,266042 |
| |
| 基金项目： | 国家自然科学基金项目(20375020)，青岛市自然科学基金项目(042JZP8) |
| |
| 摘 要： | 以金电极为基体电极,采用巯基乙醇自组装法制备了巯基乙醇自组装膜修饰电极,再以碳二亚胺为交联剂用控制电位共价键合法将DNA固定在巯基乙醇单分子层上形成了基因修饰电极。以亚甲蓝为电化学杂交指示剂,采用循环伏安法、微分脉冲伏安法等电化学方法对基因电化学传感器进行了表征。结果表明,该方法能够将DNA稳定地固定于电极表面,可用于制备自组装修饰基因电化学传感器。
|
| 关 键 词： | DNA 自组装单分子层 亚甲蓝 电化学 |
| 文章编号： | 1672-6987(2005)03-0193-04 |
| 修稿时间： | 2004-11-17 |
Preparation and Characterization of Mercaptoethanol Self-assembled Electrochemical Gene Sensor Designed by Constant Potential Covalently Bonding Method |
| |
| LI Qing-jun,JIAO Kui,SUN Wei,HU Xuan. Preparation and Characterization of Mercaptoethanol Self-assembled Electrochemical Gene Sensor Designed by Constant Potential Covalently Bonding Method[J]. Journal of Qingdao University of Science and Technology:Natutral Science Edition, 2005, 26(3): 193-196 |
| |
| Authors: | LI Qing-jun JIAO Kui SUN Wei HU Xuan |
| |
| Abstract: | A self-assembled monolayer(SAM) modified electrode was obtained by mercaptoethanol self-assembling on gold electrode. Then DNA was covalently bonded onto the SAM by using N-(3-dimethylamino)propyl-N′-ethylcarbodiimide hydrochloride (EDC) as cross linker. Using methylene blue as the indicator, the electrode was characterized by electrochemical methods such as cyclic voltammetry and differential pulse voltammetry. The results indicated that DNA was stably immobilized on the SAM/Au electrode surface, providing a new method to prepare self-assembled DNA modified electrode. |
| |
| Keywords: | DNA self-assembled monolayer methylene blue electrochemical method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
