Optimization of subcritical fluid extraction of seed oil from Nitraria tangutorum using response surface methodology |
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Authors: | Zenggen Liu Lijuan Mei Qilan Wang Yun Shao Yanduo Tao |
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Affiliation: | 1. Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China |
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Abstract: | Subcritical fluid extraction (SFE) technology was used to extract oil from Nitraria tangutorum seed. The best possible combination of extraction parameters was found using response surface methodology (RSM) in a three-variable, three-level Box-Behnken experimental design (BBD). The optimum extraction parameters were an extraction time of 40 min, an extraction pressure of 0.60 MPa, an extraction temperature of 44 °C and a raw material particle size of 0.45 mm. Conventional solvent extraction and supercritical CO2 fluid extraction were comparatively used. The yield of seed oil obtained using SFE was 12.92%, which was similar to or higher than the other methods. The chemical compositions of the seed oil, determined by GC–MS, indicate that its unsaturated fatty acids content was 97%. SFE proved to be an effective technique for extracting oil from N. tangutorum. |
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Keywords: | Subcritical fluid extraction Response surface methodology Seeds oil GC&ndash MS Nitraria tangutorum Bobr |
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