Development of laser scanning microscopy using a near ultraviolet laser

We have developed a legitimate fluorescence con-focal scanning microscope (CLSM) using a near ultraviolet (UV) laser. This system has almost no chromatic aberration from the near UV region to the visible region (350–600 nm), and the objectives are designed as water-immersion type. Therefore this system provides the high-quality fluorescence image excited by the near UV laser, and high-quality image of deep points in a sample.