| 金枪鱼制品中几种DNA提取方法的效果比较 |
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| 引用本文： | 许文杰,熊雄,黄曼虹,李壹,操敏,陆利霞,熊晓辉.金枪鱼制品中几种DNA提取方法的效果比较[J].现代食品科技,2021,37(7):57-65. |
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| 作者姓名： | 许文杰 熊雄 黄曼虹 李壹 操敏 陆利霞 熊晓辉 |
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| 作者单位： | 南京工业大学食品与轻工学院,江苏南京 211816;南京工业大学食品与轻工学院,江苏南京 211816;江苏省食品安全快速检测公共技术服务中心,江苏南京 211816 |
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| 基金项目： | 国家自然科学青年基金项目（31701688） |
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| 摘 要： | 金枪鱼种类繁多,市场上以次充好、以假乱真的现象时有发生.基于DNA的检测技术已经被广泛用于金枪鱼制品品种掺假检测.而DNA提取对DNA检测技术的运用至关重要.本研究以28份深加工金枪鱼产品为样本,采用3种DNA提取方法(SDS法以及2种市售试剂盒)进行DNA提取,并对DNA的质量、得率、PCR扩增及方法的可操作性等方面...
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| 关 键 词： | 金枪鱼 DNA提取 PCR扩增 品种掺假 |
| 收稿时间： | 2020/9/25 0:00:00 |
Comparison of Extraction Effect of Several DNA Extraction Methods in Tuna Products |
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| XU Wen-jie,XIONG Xiong,HUANG Man-hong,LI Yi,CAO Min,LU Li-xi,XIONG Xiao-hui.Comparison of Extraction Effect of Several DNA Extraction Methods in Tuna Products[J].Modern Food Science & Technology,2021,37(7):57-65. |
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| Authors: | XU Wen-jie XIONG Xiong HUANG Man-hong LI Yi CAO Min LU Li-xi XIONG Xiao-hui |
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| Affiliation: | (1.College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China); (1.College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China)
(2.Jiangsu Public Technical Service Center for Rapid Detection of Food Safety, Nanjing 211816, China) |
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| Abstract: | There are many types of tuna, shoddy and fake tuna products are found in the market from time to time. DNA-based detection technologies have been widely used in the detection of adulteration of tuna products. DNA extraction is crucial to the application of DNA detection technology. In this study, 28 deep-processed tuna products were used as the experimental samples. Three different DNA extraction methods (SDS method and two commercially available kits) were compared in terms of DNA quality, yield, PCR amplification and method operability. The results showed that all the three methods can be used for DNA extraction from most tuna products. Compared with the other two commercially available kits, the SDS method led to a lower DNA yield with a higher purity and a A260-to-A280 ratio (1.89), less salt residue, lower inhibition in real-time fluorescent quantitative PCR and relatively low cost, which meets the demand for adulteration detection of tuna products. |
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| Keywords: | tuna DNA extraction PCR amplification variety adulteration |
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