A Simplified Myosin Preparation from Marine Fish Species |
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| Authors: | CELINA B MARTONE LILIANA BUSCONI EDUARDO J FOLCO RAÚL E TRUCCO JORGE J SANCHEZ |
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| Affiliation: | The authors are affiliated with Instituto Nacional de Tecnologia Industrial, INTI, Centro de Investigaciones de Tecnologia Pesquera, CITEP, Marcelo T. de Alvear 1168. 7600-Mar del Plata, Argentina. Author Trucco is Career Investigator of the Consejo Nacional de lnvestigaciones Cientificas y Técnicas (Argentina). Author Martone is affiliated with Consejo Nacional de Investigaciones Cientificas y Tecnicas. |
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| Abstract: | A simple and rapid method for the purification of fish myosin with high yield was developed. The method consisted of washing chopped fish muscle with a 0.1M KCl solution, an extraction of myosin with a 0.45M KCl solution containing ATP, and steps of reprecipitation and redissolution by changes in ionic strength. Actin can easily be obtained as a component of the myosin preparation. |
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