| HPLC-CL柱后同时检测葡萄酒中的有机酸 |
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| 引用本文： | 吴永平,高智席,王满力.HPLC-CL柱后同时检测葡萄酒中的有机酸[J].酿酒科技,2007(1):99-101. |
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| 作者姓名： | 吴永平 高智席 王满力 |
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| 作者单位： | 1. 遵义师范学院化学系,贵州,遵义,563002
2. 贵州大学化工学院,贵州,贵阳,550003 |
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| 摘 要： | 基于在酸性介质中高锰酸钾直接氧化柠檬酸、苹果酸和琥珀酸产生化学发光,而盐酸氨基脲能够显著增强该体系的发光强度,建立了反相高效液相色谱分离柱后化学发光检测柠檬酸、苹果酸和琥珀酸的方法,并成功应用于葡萄酒中有机酸的测定.
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| 关 键 词： | 葡萄酒 高效液相色谱 化学发光 有机酸 同时检测 葡萄酒 有机酸 Chemiluminescence HPLC Wine Grape Organic Acids 测定 应用 方法 发光检测 化学发光 分离柱 高效液相色谱 反相 发光强度 体系 增强 盐酸氨基脲 |
| 文章编号： | 1001-9286（2007）01-0099-03 |
| 修稿时间： | 2006-10-16 |
Detection of Organic Acids in Grape Wine by HPLC and Post-column Chemiluminescence |
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| WU Yong-ping,GAO Zhi-xi,WANG Man-li.Detection of Organic Acids in Grape Wine by HPLC and Post-column Chemiluminescence[J].Liquor-making Science & Technology,2007(1):99-101. |
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| Authors: | WU Yong-ping GAO Zhi-xi WANG Man-li |
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| Affiliation: | 1, Chemistry Department of Zunyi Normal College, Zunyi, Ggizhou 563002; 2. Chemical Engineering College of Guizhou University, Guiyang, Guizhou550003, China |
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| Abstract: | Bemuse KMnO4 could oxidize citric acid, malic acid and amber acid directly in sulfuric acid medium to produce chemiluminescence and semicarbazide hydrocbloride could evidently enhance the chemiluminescent intensity, the method of HPLC coupled with CL was developed to detect organic acids in grape wine. Such method had been used sucessfully in the detection of organic acids in grape wine. |
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| Keywords: | grape wine high-performance liquid chromatography (HPLC) chemiluminescence organic acids |
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