Confocal laser scanning microscope evaluation of early bacterial colonization on zirconium oxide and titanium surfaces: An in vivo study |
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Authors: | Min Wang Yi Bai Hui Yang Haixiao Zou Haibin Xia Yining Wang |
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Affiliation: | 1702. Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China 2702. Department of Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China 3702. Department of Stomatology, Shanghai Punan Hospital, Shanghai, 200125, China 4702. Department of Prosthodotics, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China
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Abstract: | To investigate the bacterial colonization on zirconium oxide and titanium surfaces in vivo quantitatively using a confocal laser scanning microscope (CLSM). Ten samples of zirconium oxide ceramic and commercially pure titanium were fabricated and polished using silicon carbide abrasive paper. One sample from each group was evaluated topographic pattern under a scanning electron microscope. One sample from each group was to evaluate roughness using a profilometer. Eight volunteers were selected. The samples were cemented at the buccal surfaces of upper first molars. All samples were removed after 48 hours, immersed in SYTO-9 and propidium iodide fluorescent to stain for adherent bacteria and observed with CLSM. Fewer bacteria were observed in zirconia group than titanium group. However, there was no statistical difference between two groups. The experimental results demonstrate that zirconium oxide may be considered as a promising material for dental implant abutments. |
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