吡哌酸锌对实验性胃溃疡的保护作用及其机制

目的: 研究吡哌酸锌对实验性胃溃疡的保护作用及其机制。 方法: 采用大鼠幽门结扎性胃溃疡、大鼠水浸应激性胃溃疡、大鼠乙酸烧灼性胃溃疡和小鼠利血平性胃溃疡四种模型进行实验。每种模型实验动物均衡随机分为 5 组, 即羧甲基纤维素钠(CMC) 阴性对照组、西米替丁(0.5 mg·kg^-1 ) 阳性对照组、吡哌酸锌(0.025、0.05、0.1 g·kg^-1 ) 3 个剂量组(小鼠剂量加倍), 灌胃给药, 每天一次, 连续 5 d,观察药物对实验性胃溃疡的保护作用。同时测定幽门结扎性胃溃疡各组大鼠胃液量、总酸排出量、胃蛋白酶活性和胃壁粘液量。结果: 吡哌酸锌能明显抑制 4 种实验性胃溃疡的形成, 并呈剂量依赖性。 吡哌酸锌 0.1 g·kg^-1剂 量组能 降低 胃液 量 (P<0.05), 吡哌酸锌 0.025、0.05、0.1 g·kg^-1 3 个剂量组均能减少总酸排出量(P<0.01);均能显著降低胃蛋白酶活性(P<0.01), 并显著增加胃壁粘液量(P<0.01)。 结论: 吡哌酸锌对实验性胃溃疡具有保护作用, 其机制与增强胃粘膜保护因素、减少胃粘膜的损伤因素有关。

Protective effect of Zn-pipemidate on experimental gastric ulcer and its mechanism

AIM: To study protective effest of Zn-pipemidate on experimental gastric ulcer and its mecha-nism. METHODS: Pylous-ligated, stess-induced, acetic acid-induced and reserpine-induced gastric ulcers in rats or mice were used in experiment.Rats or mice were randomly divided into five groups including car-boxymethylcellulose (CMC), cimetidine and three doses of Zn-pipemidate (0.025, 0.05, 0.1 g·kg^-1 ).The drugs were given ig, once a day, for 5d.Gastric juice, out-put of pan-acid, pepsin activity and the volume of mucusgas-tric wall were determined in pylorus-ligated gastric ulcer in rats.RESULTS: Zn-pipemidate could inhibited the formation of all gastric ulcer models in a dose-dependent manner.All of the three doses of Zn-pipemidate groups could reduce out-put of pan-acid (P<0.01) and pepsin activity, and increase the volume of mucus gastric wall (P<0.01).CONCLUSION: Zn-pipemidate can pro-tect experimental gastric ulcer.Its mechanism is related to increasing protective factors and reducing damaging factors of gastric mucosa.