苯妥英钠对鼠脑缺血再灌注时血浆细胞因子及内皮素的影响

目的 探讨苯妥英钠对脑缺血再灌注后TNFα、IL-Ⅰβ 和ET₁的影响。方法 采用线栓法制备大鼠局灶性脑缺血再灌注模型, 用放射免疫方法检测脑缺血再灌注大鼠血浆TNFα、IL-Ⅰβ、ET₁的变化, 以及苯妥英钠对TNFα、IL-Ⅰβ、ET₁ 含量的干预作用。结果 缺血再灌组与假手术组相比, 血浆TNFα、IL-Ⅰβ、ET₁ 含量明显增加, 应用苯妥英钠各剂量组TNFα、IL-Ⅰβ、ET₁水平均降低。结论 TNFα、IL-Ⅰβ、ET1 参与了局灶性脑缺血再灌注损伤的病理过程, 苯妥英钠通过降低TNFα、IL-Ⅰβ、ET₁ 的含量能发挥一定的脑保护作用。

Effects of phenytoin on plasma cytokines and endothelin during brain ischemic reperfusion in rats

Aim In this study, cytokines and end othelins were investigated to determine the changes of TNFα, IL- Ⅰβ and ET1 after brain ischemic reperfusion and the effects of phenytoin sodium on TNFα, IL-Ⅰβ and ET₁. Methods The animal models of the focal middle cerebral arteral is chemic reperfusion were established in Wistarrats. Phenytoin sodium was administered by intravenous injection. The plasma levels of TNFα, IL-Ⅰβ and ET₁ were measured by radioimmunoassay. Rusults The levels of TNFα, IL-Ⅰβ and ET₁ in plasma were elevated after 1 h brain ischemia and 1 h reperfusion in rats. Phenytoin sodium could significantly reduce the content of TNFα, IL-Ⅰβ and ET1 in plasma. Conclusion The results indicate that TNFα, IL-Ⅰβ and ET1 participate in cerebral ischemia reperfusion lesion, which can be reduced by phenytoin sodium. Phenytoin sodioum may protect nerve cells from injury through inhibiting TNFα, IL-Ⅰβ and ET₁ production.