| 阿米替林对谷氨酸损伤中枢神经细胞的保护作用 |
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| 引用本文： | 徐庆荣.阿米替林对谷氨酸损伤中枢神经细胞的保护作用[J].金属学报,2002,7(1):24-26. |
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| 作者姓名： | 徐庆荣 |
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| 作者单位： | 山东省滨州医学院, 滨州 256603 |
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| 摘 要： | 目的: 研究阿米替林(Ami) 对谷氨酸损伤培养大鼠皮层神经细胞的保护作用。 方法: 分离培养15~ 18 d 胎龄的大鼠神经细胞, 加入谷氨酸(Glu), 造成神经细胞的损伤, 测定乳酸脱氢酶、一氧化氮、 丙二醛及超氧化物歧化酶的含量。观察谷氨酸对神经细胞的损伤作用及阿米替林的保护作用。 结果: 加入谷氨酸后, 神经细胞出现明显损伤性变化, 死亡率升高, 培养上清液中乳酸脱氢酶(LDH)、 一氧化氮(NO) 含量增加, 细胞匀浆中丙二醛(MDA) 生成增加, 超氧化物歧化酶(SOD) 含量明显减少。Ami 10-8~10-6 mol·L-1 能不同程度地减轻上述损伤性变化。结论: Ami 对谷氨酸所致的神经细胞损伤具有保护作用, 其作用机制可能与抗脂质过氧化作用有关。
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| 关 键 词： | 阿米替林 谷氨酸 大脑皮层神经细胞 细胞培养 |
| 收稿时间： | 2001-09-03 |
| 修稿时间： | 2001-10-11 |
Protective effects of amitriptyline on glutamate-induced neurotoxicity in cerebral neuronal cultures |
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| XU Qing-Rong.Protective effects of amitriptyline on glutamate-induced neurotoxicity in cerebral neuronal cultures[J].Acta Metallurgica Sinica,2002,7(1):24-26. |
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| Authors: | XU Qing-Rong |
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| Affiliation: | Binzhou Medical College, Binzhou 256603 |
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| Abstract: | AIM: To study the protective effects of amitriptyline (Ami) on glutamate-induced neurotoxicity in cultured rat cerebral cortical neurons.METHODS: Cor-tical neurons of fetal rat were cultured in vitro. The pro-tective effects of Ami on glutamate-induced neurotoxicity were observed.RESULTS: Exposure of rat fetus cerebral cells to Glu medium developed a neurotoxicity, expressed in the increase of LDH, NO andMDA, and the decrease of activity of SOD, as well as the development of morpho-logical injury. Ami (10-6, 10-7, 10-8 mol·L-1 ) signifi-cantly protected neurons against above demage.CONCLUSION: Ami can prevent rat cerebral neurons injury from the toxicity of Glu. |
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| Keywords: | amitriptyline glutamate cerebral corti-cal neurons cell culture |
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