氯苄四氢小檗碱对抗H2O2引起无血清培养的血管内皮细胞凋亡及坏死

目的: 研究氯苄四氢小檗碱对过氧化氢(H₂O₂)引起无血清培养的小牛主动脉血管内皮细胞的凋亡、增殖、坏死的影响。方法: 通过体外细胞培养, 以噻唑兰(MTT) 法测定细胞存活率;用流式细胞术检测细胞DNA 含量及凋亡细胞百分率、增殖率;DNA 琼脂糖凝胶电泳法观察细胞凋亡过程中DNA 断裂程度。结果: H₂O₂ 诱导无血清培养的内皮细胞凋亡, 氯苄四氢小檗碱可显著降低内皮肌细胞中凋亡细胞百分率, 并抑制其增殖, 也减少凋亡细胞DNA 断裂, 同时也减少其引起的平滑肌细胞坏死。结论: 氯苄四氢小檗碱可对抗H₂O₂ 引起的无血清培养的血管平滑肌细胞凋亡、增殖及坏死。

Inhibition of chlorobenzyl-tetrahydroberberine on the H2O2-induced apoptosis and proliferation of endothelial cells

AIM: To examine the inhibition of chlorobenzyl-tetrahydroberberine (BTHB) on the H₂O₂-induced apoptosis and proliferation of endothelial cells(ECs) in the serum deprivation medium.METHODS: The DNA content and percentage of apoptosis were measured by flow cytometry;the DNA fragmentation was analyzed by agarose gel electrophorsis;the viability percentage of cells was measured using MTT assay.RESULTS: The apoptosis and proliferation of ECs occuredin serum-free medium (18 h) containing H₂O₂.When the ECs had been treated with BTHB, the percentage of ECs apoptosis and proliferation was markedly decreased, and the degraded fragments of DNA obviously decreased.BTHB (10-5, 10-6, 10-7, 10-8mol·L^-1) could partly protect cells from necrosis induced by H₂O₂.CONCLUSION: BTHB can prevent ECs from apoptosis, proliferation and necrosis induced by H₂O₂ in free-serum medium.