蝎毒组分Ⅲ对人头颈部鳞状细胞癌细胞生长的影响

目的 观察蝎毒组分Ⅲ(SVC-Ⅲ)对人头颈部鳞状细胞癌细胞株(AGZY-973)生长的影响,试图找出SVC Ⅲ抑制鳞状细胞癌的最佳浓度。方法 采用噻唑蓝(MTT)法检测经不同浓度SVC-Ⅲ 作用下AGZY-973 生长抑制率。结果 SVC Ⅲ能抑制该细胞的生长,SVC-Ⅲ可导致AGZY-973 细胞代谢MTT 的能力显著低于对照组,生长抑制率达68.96%(P <0.01)。当SVC Ⅲ的浓度<40 mg·L^-1时,剂量效应关系明显;而SVC-Ⅲ的浓度>40 mg·L^-1时无剂量效应关系。5-Fu 治疗组生长抑制率达55.27% P <0.01)。结论 SVC-Ⅲ是存在于东亚钳蝎蝎毒中的一种抗肿瘤成分,能够抑制AGZY-973 的生长,并在一定浓度范围内存在剂量效应关系。

Effect of the component Ⅲ in scorpion venom crude extracted from buthus martensii karsch on the human jugular squamous cell carcinoma AGZY-973cell line

AIM: To observe the cytotoxicity effect of scorpion venom crude(SVC-Ⅲ)on the human jugular squamous cell carcinoma(AGZY-973)and to find a properly consistence of SVC of the growth inhibiting to AGZY-973.METHODS: MTT colorimetric method was used to study the growth inhibition rate(GIR)in different condition.RESULTS: The ability to metabolize MTT of AGZY-973 cells was lower than control distinctly after the cells were treated by SVC-Ⅲ.The growth of AGZY-973 cells was inhibited obviously by SVC Ⅲ and GIR was reached 68.96%(p<0.01).The dose-response relationshipwas unclear when consistence were more than 40mg·L^-1,while the dose-response relationshipwas clear when dose was in 10 -40 mg·L^-1.The growth inhibition rate(GIR)of 5-Fu reached 55.27%(p<0.01).CONCLUSION: SVC is one kind of effective antineoplastic components in the venom of buthus martensii scorpion.It possesses growth inhibition on AGZY-973 cells.The properly consistence of SVC of the growth inhibition to AGZY-973 cells probably is 40 mg·L^-1.