果糖二磷酸钠镁对缺血突触体乳酸含量及ATP酶活性的影响

目的 研究果糖二磷酸钠镁(FDPM)对缺血突触体乳酸含量及ATP 酶活性的影响,以探讨其脑保护作用机制。方法 制备正常大鼠脑突触体,氧糖剥夺培养建立缺血突触体模型,分别加1.3 mmol·L^-1硫酸镁,4.0 mmol·L^-11,6-二磷酸果糖(FDP)及1.3mmol·L^-1 果糖二磷酸钠镁共培养60 min,测定突触体乳酸含量及Na⁺-K⁺ATP 酶和Ca²⁺-Mg²⁺ATP 酶活性。结果 FDPM 可明显降低缺血突触体乳酸含量,升高Na⁺-K⁺ATP 酶和Ca²⁺-Mg²⁺ATP 酶活性(P <0.05),其作用强于FDP 的作用。结论 FDPM保护脑缺血的作用机制可能与其改善脑缺血后能量代谢,减轻脑组织酸中毒状态有关。

Effects of sodium magnesium fructose diphosphate on lactate concentration and ATPase activity of ischemic synaptosome

AIM: To study the effects of sodium magnesium fructose diphosphate(FDPM)on lactate concentration and ATPase activity of ischemic synaptosome,so as to explore the protective mechanisms of FDPM on cerebral ischemia.METHODS: Synaptosome prepared from normal rat brain was cultured with oxygen and glucose deprived to establish the model of ischemic synaptosome.1.3 mmol·L^-1magnesium sulfate,4.0mmol·L^-1 fructose-1,6-diphosphate(FDP)and 1.3 mmol ·L^-1 FDPM were incubated with the ischemic synaptosomes separately.After 60min,the lactate concentration as well as the activities of Na⁺-K⁺ATPase and Ca²⁺-Mg²⁺ATPase were detected.RESULTS: FDPM reduced the lactate concentration,raised the activities of Na⁺-K⁺ATPase and Ca²⁺-Mg²⁺ATPase of ischemic synaptosomes(p<0.05).The effects of FDPM were stronger than those of FDp(p<0.05).CONCLUSION: FDPM may protect cerebral ischemia by improving the energy metabolism,and mitigating acidosis state of brain tissue.