猪苓多糖对膀胱癌细胞钙离子浓度的影响

目的 观察猪苓多糖对人T24 膀胱癌细胞内钙离子(Ca²⁺) 浓度的影响, 探讨猪苓多糖抗癌机理。方法 体外培养人T24 细胞经钙荧光指示剂(calciumcrimson-AM) 负载后, 用激光扫描共聚焦显微镜测定猪苓多糖作用前后细胞内Ca²⁺随时间的变化。结果 猪苓多糖低剂量(0.2 mg·L^-1) 使T24 细胞内Ca²⁺降低, 高剂量(>1 mg·L^-1) 使T24 细胞内Ca²⁺升高, 最后维持在高钙水平上。猪苓多糖主要促进细胞核内Ca²⁺升高, 细胞浆Ca²⁺无明显改变。结论 猪苓多糖可引起T24 细胞内Ca²⁺水平的显著变化, 而且这种变化主要表现在细胞核内, 提示猪苓多糖可能有诱导细胞凋亡的作用。

Dynamic observation of the effects of polyporus polysaccharide on intracellular calcium in cultured human T24 cells with laser scanning confocal microscopy

Aim To investigate the relationship between polypo rus polysaccharide (PUPS) and changes of int racellular calcium in human T24 bladder cancer cells. Methods Human T24 cells were cultured with PUPS and intracellular calcium level was measured with the fluorescent Ca²⁺ indicato r calcium crimson- AM and the laser scanning confocal microscopy.Results After human T24 cells were treated with a low dose of PUPS (0.2 mg·L^-1), the int racellular calcium was decreased; while with PUPS (> 1.0 mg·L^-1), the int racellular calcium became increased, and kept on ahigher level.Conclusion PUPS participates in the process of calcium message transduct ion.