黄芪总提物对肝癌细胞生长、甲胎蛋白分泌及γ-GT 活性的影响1

目的 研究黄芪总提物(TEA) 对肝癌细胞生长、甲胎蛋白(AFP) 分泌及γ-谷氨酰转移酶(γ-GT)活性的影响。方法 以两种人肝癌细胞株HepG2 细胞和Bel-7404 细胞分别与TEA(5 ~ 160 mg · L^-1)共培养6 d, 利用MTT 比色、ELISA 及细胞分化检测等方法。结果 TEA(5 ~ 160 mg ·L^-1)可抑制两种人肝癌细胞增殖, 并且明显降低HepG2 细胞高水平分泌AFP 。T EA(20、40 和80 mg ·L^-1)可抑制肝癌HepG2 细胞的γ-GT 活性, 升高白蛋白(ALB) 含量。TEA(40 和80 mg · L^-1)亦可抑制肝癌Bel-7404 细胞的γ-GT 活性, 并升高ALB 含量。结论 TEA 具有抑制肝癌生长、AFP分泌及γ-GT 活性的作用。

Effects of the total extract of astragalosides on the growth,AFP secretion and 7-GT activity of human hepatoma cells1

Aim To study the effect of the total extract of astragalosides (TEA) on the growth AFP secretion and 7~GT activity of human hepatoma cells in vitro. Methods The human hepatoma HepG2 and Bel-7404 cells were cultured with TEA 5 ~ 160 mg· L^-1 for 6 days. Anti hepatoma activity was measured by MTT method. AFP was assaysed by ELISA and 7~GT was determined by enzyme substrate method. Results The growth of the human hepatoma HepG2 and Bel-7404 cells and the secretion of AFP of HepG2 cells were reduced significantly by TEA 5 ~ 160 mg · L^-1. The YGT activity of HepG2 cells was inhibited and its ALB content was increased by TEA 20,40 and 80 mg · L^-1. The 7~GT activity of Bel-7404 cell was inhibited and its ALB content was increased by TEA 40 and 80 mg· L^-1. Conclusion These results suggest that TEA has inhibitory effects on the growth, AFP secretion and YGT activity of human hepatoma cells.