黄芪总提物对大鼠肝星状细胞增殖及产生胶原的影响

目的 研究黄芪总提物(TEA) 对体外肝星状细胞增殖和产生胶原的影响。方法 采用IV 型胶原酶灌流分离大鼠肝星状细胞进行原代培养, 用CCl₄急性损伤的大鼠肝枯否细胞条件培养基(KCCM)刺激肝星状细胞, 用³H-TdR 和³H-Proline 的掺入法分别检测肝星状细胞的增殖和胶原的产生。结果 TEA (5 ~ 40 mg·L^-1) 可明显降低由KCCM 刺激的肝星状细胞的增殖和胶原的产生。结论 TEA对体外激活的肝星状细胞的增殖和产生胶原有明显抑制作用, 该作用可能是TEA 抗肝纤维化的机理之一。

Effects of total extract of astragalus (TEA) on the proliferation and the production of collagen of hepatic stellate cells

Aim To study the effect s of total extract of astragalus(TEA) on activating the primary cultured hepatic stellate cells (HSC) and synthesizing collagen. Methods Livers of adult rats were perfused by pronase E and collagenase in situ, HSC and Kupf fer cell (KC) were isolated by centrifugation with 18 % Nycodenz. The subcultured HSC w ere induced by Kupffer cell conditioned-medium(KCCM) and were incubated with 5 ~ 80 mg·L^{-1 TEA for 6 days. The proliferation of the cells was measured by 3H-TdR and the production of collagen by 3H-Proline. Results TEA (10 ~ 40 mg·L-1) could suppress the proliferation of hepatic stellate cells and TEA (40 ~ 80 mg·L-1) could suppress the production of collagen. Conlusion The suppression of hepatic stellate cell proliferation and the collagen’ s production by the TEA may be one of the mechanisms for depressing the hepatic fibrosis by the TEA.}