A Cotinine in Freeze-Dried Urine Reference Material |
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Authors: | Lane C. Sander Gary D. Byrd |
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Affiliation: | National Institute of Standards and Technology, Gaithersburg, MD 20899 |
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Abstract: | A cotinine in freeze-dried urine reference material (RM 8444) was prepared at three concentrations: (1) a “blank” level typical of nonsmokers with no exposure to cigarette smoke, (2) a “low” level corresponding to nonsmokers with passive exposure to side-stream smoke, and (3) a “high” level typical of smokers. Low- and high-level materials were prepared gravimetrically from pooled urine by the addition of appropriate amounts of cotinine Perchlorate. Cotinine was determined by GC-MS using cotinine-d3) as an internal standard. No evidence for sample inhomogeneity was observed. This reference material will fulfill a need for a urine-based standard to assist in the validation of field methods used for assessing exposure to cigarette smoke. |
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Keywords: | cotinine cotinine Perchlorate GC-MS passive smoking side stream smoke standards tobacco |
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