In vivo evaluation of composites of PLGA and apatite with two different levels of crystallinity |
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Authors: | Tohru Hayakawa Chihiro Mochizuki Hiroki Hara Fei Yang Hong Shen Shenguo Wang and Mitsunobu Sato |
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Affiliation: | (1) Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo, 2-870-1, Sakaecho-nishi, Matsudo, Chiba 271-8587, Japan;(2) Coordination Engineering Laboratory, Faculty of Engineering, Kogakuin University, 2665-1, Nakano, Hachioji, Tokyo 192-0015, Japan;(3) PPCL, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China |
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Abstract: | The cortical bone response towards poly(lactide-co-glycolide) (70/30) (PLGA) (70/30)/apatite complex scaffolds with different
levels of crystallinity was investigated. Apatite with different levels of crystallinity, Ca-deficient hydroxyapatite (CDHA),
which has a low crystallinity, and a mixture of carbonated hydroxyapatite (CHA) and CDHA, which has a higher crystallinity,
were prepared from an aqueous mixture of Ca-EDTA complex, H2O2, H3PO4, and NH4OH. Two porous PLGA(70/30)/apatite composite scaffolds, composite scaffold A (containing low crystallinity CDHA) and composite scaffold B (containing the higher crystallinity CHA/CDHA mixture), were prepared. Afterwards, pure porous PLGA and the two composite
scaffolds were implanted into the cortical bone of rabbit tibiae for 12 weeks. High-resolution microfocus X-ray computed tomography
and histological examinations revealed a better bone response for composite scaffold A compared with PLGA and composite scaffold B. For composite scaffold A, the original bone defect was almost filled with new bone. Quantitative analysis revealed that composite scaffold A produced a significantly greater amount of new bone. The present study demonstrated that the level of apatite crystallinity
influences bone response. A PLGA/apatite porous composite with a low level of apatite crystallinity shows promise as a bone
substitute or scaffold material for bone tissue engineering. |
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