| 产耐温漆酶菌的筛选及培养基优化 |
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| 引用本文： | 吴振强,秦鹏,王菊芳,梁世中.产耐温漆酶菌的筛选及培养基优化[J].西北轻工业学院学报,2009,27(1):68-73. |
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| 作者姓名： | 吴振强 秦鹏 王菊芳 梁世中 |
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| 作者单位： | 吴振强,WU Zhen-qiang(华南理工大学生物科学与工程学院,广东,广州,510640;华南理工大学制浆造纸工程国家重点实验室,广东,广州,510640);秦鹏,王菊芳,梁世中,QIN Peng,WANG Ju-fang,LIANG Shi-zhong(华南理工大学生物科学与工程学院,广东,广州,510640)
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| 基金项目： | 纸浆造纸工程国家重点实验室开放基金 |
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| 摘 要： | 采用愈创木酚CPDA平板法对由广州地区多个不同场所采集的土样进行了菌种分离纯化，筛选出1株产耐温漆酶的菌株Tr0702，通过形态学观察初步判断该菌为木霉属．该菌所产漆酶的最适反应温度为65℃，在70℃下保温60min后残余酶活保留60％以上；发酵培养基经响应面法优化后关键参数如下：可溶性淀粉44．40g／L、吐温（80）9．00g／L、愈创木酚15．04mmol／L，以此优化培养基发酵酶活可达50．15U／mL
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| 关 键 词： | 漆酶 耐温 筛选 响应面法 优化 |
SCREENING OF THERMO TOLERANT LACCASE PRODUCING STRAIN AND ITS MEDIUM OPTIMIZATION |
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| WU Zhen-qiang,QIN Peng,WANG Ju-fang,LIANG Shi-zhong.SCREENING OF THERMO TOLERANT LACCASE PRODUCING STRAIN AND ITS MEDIUM OPTIMIZATION[J].Journal of Northwest University of Light Industry,2009,27(1):68-73. |
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| Authors: | WU Zhen-qiang QIN Peng WANG Ju-fang LIANG Shi-zhong |
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| Affiliation: | WU Zhen-qiang, QIN Peng, WANG Ju-fang, LIANG Shi-zhong( 1. College of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510640, China; 2. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology,Guang- zhou 510640, China) |
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| Abstract: | By using CPDA plate with O-methoxypheno, a laccase-producing strain, Tr0702 was isolated from several soil samples which were collected from different places in Guangzhou. The laccase produced by Tr0702 showed tolerance of high temperature, which still remained more than 60% enzymatic activity after incubated in 70 ℃ for about 60 minutes. The optimal catalytic temperature was 65℃. The results of reponse surface methodology showed that the optimal components for fermentation medium were soluble starch 44. 40 g/L, tween 80 9.00 g/L, and O-methoxypheno 15. 04 mmol/L. After optimization, the laccase activity reached 50. 15 U/mL. |
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| Keywords: | laccase thermal tolerant screening response surface methodology optimization |
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