| Alcaligenes sp.碱性脂肪酶产生条件及酶学性质的研究 |
| |
| 引用本文： | 洪骏,夏黎明.Alcaligenes sp.碱性脂肪酶产生条件及酶学性质的研究[J].食品与发酵工业,2002,28(11):8-11. |
| |
| 作者姓名： | 洪骏 夏黎明 |
| |
| 作者单位： | 浙江大学材料与化工学院化学工程与生物工程系,杭州,310027 |
| |
| 摘 要： | 对Alcaligenessp .产碱性脂肪酶的工艺条件进行了研究。结果表明 ,适宜产酶培养基为 :3 %黄豆粉 ,1%果糖 ,0 .2 %K3 PO4,1%NaNO3 ,0 .6%柠檬酸钠 ,0 .0 3 %FeSO4,0 .5 %聚乙烯十八烷基醚 ;最佳培养条件为 2 5℃ ,初始pH9.0。脂肪酶活力可高达 12 0IU/mL。该酶在5 5℃以下 ,pH6.0～ 10 .5范围内稳定 ;酶反应适宜温度为 40℃ ,适宜pH9.0。该酶制剂在洗涤和制革工业中有良好的应用前景。
|
| 关 键 词： | Alcaligenessp. 碱性脂肪酶 生产 酶学性质 |
| 修稿时间： | 2002年4月5日 |
Studies on Production Conditions and Enzymatic Properties of Alkaline Lipase by Alcaligenes sp. |
| |
| Hong Jun,Xia Liming.Studies on Production Conditions and Enzymatic Properties of Alkaline Lipase by Alcaligenes sp.[J].Food and Fermentation Industries,2002,28(11):8-11. |
| |
| Authors: | Hong Jun Xia Liming |
| |
| Abstract: | The main factors affecting the lipase production by Alcaligenes sp. were studied. The results showed that the suitable medium for lipase producing was: 3% of soybean powder, 1% of fructose, 0.2% of K 3PO 4, 0.6% of sodium citrate, 0.03% FeSO 4·7H 2O, 0.5% of polyethylene stearyl ether. Under optimal culture conditions 25℃ and pH 9.0, the maximal lipase activity could reach 120?IU/mL. The enzyme was stable under 55℃ and pH 6.0～10.5, with optimal temperature and pH for enzymatic reaction were 40℃ and pH 9.0, having a good potential application in detergent and leather industry. |
| |
| Keywords: | Alcaligenessp |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
