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HPLC测定参芪片中人参皂苷Re的含量
引用本文:王晓林,李蕊,徐状.HPLC测定参芪片中人参皂苷Re的含量[J].吉林化工学院学报,2010,27(1):30-32.
作者姓名:王晓林  李蕊  徐状
作者单位:1. 吉林化工学院,化学与制药工程学院,吉林,吉林,132022
2. 中油吉化集团总医院,神经内科,吉林,吉林,132022
摘    要:建立了测定参芪片中人参皂苷Re含量的HPLC法.采用色谱柱为AT-330C18;流速为1.0mL/min;流动相为乙腈-0.05%磷酸溶液(2080);检测波长为203nm.人参皂苷Re得到了较好的分离,在0.3486~3.486μg范围内呈良好的线性关系.本法简便快捷,结果可靠,可用于参芪片中人参皂苷Re的含量测定.

关 键 词:参芪片  人参皂苷Re  HPLC方法  含量测定

Determination of ginsenoside Re in Shenqi Tablets by HPLC
WANG Xiao-lin,LI Rui,XU Zhuang.Determination of ginsenoside Re in Shenqi Tablets by HPLC[J].Journal of Jilin Institute of Chemical Technology,2010,27(1):30-32.
Authors:WANG Xiao-lin  LI Rui  XU Zhuang
Affiliation:1. College of Chemical & Pharmaceutical Engineering;Jilin Institute of Chemical Technology;Jilin City 132022;China;2. Department of Neurology;Petrochina Jilin Petrochemical Company Hospital;Jilin 132022;China
Abstract:To develop a method for the determination of ginsenoside Re in Shenqi tablets, HPLC method was discussed in detail with the work condition as follows:AT-330 C18 as working column, and acetonitrile-O. 05% phosphoric acid (20 : 80 ) as the mobile phase, with the flow rate of 1.0 mL · min^-1 and a detection wavelength of 203 nm. Ginsenoside Re was well separated and it calibration curves were in good linearity over the average of 0. 348 6 - 3. 486 μg. The HPLC method is simple and accurate which is suitable for determining the eontents of ginsenoside Re in Shenqi tablets.
Keywords:shenqi tablets  ginsenoside Re  HPLC method  determination  
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