顶空技术测定阿昔洛韦中的残留溶剂

目的：建立阿昔洛韦中的残留溶剂乙醇、异丙醇、甲苯的分离测定方法。方法：采用Agilent-DB-1石英毛细管气相色谱柱（30mX0．53nunx5．00胁m），载气为氮气，氢火焰离子化检测器，进样口温度为180℃，检测器温度为250℃。柱温采用程序升温：初始温度为40℃。维持0min，以20℃·min^-1叫速率升至80℃，维持12min；流速为5mL·min^-1。以二甲基亚砜为样品溶剂。结果：乙醇、异丙醇、甲苯的检测限分别为0．257，O．216，O．074μg·mL^-1；定量限分别为1．026，1．020，0．520μg·mL^-1；平均回收率（n=9）分别为100．2％，100．6％，101．2％。结论：本法操作简便，灵敏度高、准确性强；适用于阿昔洛韦中残留溶剂的检测。

Study on the Analysis of Residual Solvents of Acyclovir by Head Space GC

Objective： To establish a method for the Acyclovir residual solvents （ethanol,isopropyl alcohol and toluene）by GC, Methods： Use the capillary column Agilent DB-1 （30 m×0.53 mm×5.00μm）, carrier gas is nitrogen gas, FID, injector temperature 180 ℃ and detector temperature 250 ℃. Column temperature is program; original temperature is 40 ℃, hold 0 min, promote to 80 ℃ use temperature speed is 20 ℃.min^-l, hold 12 min, flow speed is 5 mL. min^-1. The sample solvent is DMSO. Results and conclusion：The detection limit of ethanol, isopropyl alcohol and toluene are 0.257, 0.216, 0.074 μg mL^-1, the quantitation limit of ethanol,isopropyl alcohol and toluene are 1.026, 1.020,0.520 μgmL^-1. Under this conditions,baseline separations of these residual solvents could be obtained. The method was simple, rapid with high resolutions.