Experimental studies on phase equilibria of PEG-water-dextran/ammonium sulfate systems and partitioning of albumin into two water-continuous phases |
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Authors: | Bong Kyu Kim Yong-Byung Ban Jong-Duk Kim |
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Affiliation: | (1) Department of Chemical Engineering and Bioprocess ERC, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-ku, 305-701 Taejon;(2) Samsung General Chemicals Co. Ltd., Seoul, Korea;(3) National Institute of Standards and Technology(NIST), 20899 Gaithersburg, MD, USA |
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Abstract: | The clouding points and equilibrium concentrations of PEG/dextran/water and PEG /ammonium sulfate/water systems were experimentally
determined for different molecular weights of PEG by titration method and direct determination of concentrations. In phase
diagrams of PEG-water with ammonium sulfate or dextran, the addition of salt or dextran induced the phase separation of PEG-rich
phases near the PEG-lean phases having the different partitioning of PEG. The concentrations of PEG in PEG-rich phases increase
as the amounts of PEG or salt and dextran increase, while the concentrations of PEG in aqueous media decrease in any cases.
The higher the molecular weight of PEG has, the wider the two-phase regions are. In dextran DT10 systems, the partition coefficients
of egg albumin into PEG-lean phases increase with concentrations of dextran and the molecular weight of PEG. In ammonium sulfate
systems, the partitioning coefficients showed a maximum, having lower partitioning at the very high and low concentration
of salts. It is also observed that as the amounts of albumin increase, the partitioning of albumin into PEG-lean phase increases. |
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