负载活性组分的核壳结构纤维膜的制备及性能

采用同轴静电纺丝技术将蛛丝蛋白（Ss）和美洲大蠊提取物（PAE）分别负载于纳米纤维的壳层与核层。随着Ss的增加，纤维直径从350 nm降至280 nm，核层直径由120nm升至140 nm，壳层厚度由115 nm降至70 nm。Ss的加入使纳米纤维膜具有良好的机械性能和亲水性，纳米纤维膜的拉伸强度可达到4.31 MPa，溶胀率可达到150%，水蒸气透过率可达到1834 g/(m2?24h)，水接触角减小到 32.7 ?。纳米纤维膜核壳结构能够有效抑制药物突释，实现药物长效释放，7天内药物释放可达77%；纳米纤维膜能够有效抑制细菌生长，促进细胞增殖，相较于未负载Ss的纳米纤维膜，负载20%Ss的纤维膜的细胞增殖效果提高25%，说明Ss和PAE在伤口愈合过程中能够起到协同作用。

Preparation and Performance of Core-shell Structure Fiber Membrane Loaded with Active Components

Spider silk protein (Ss) and Periplaneta Americana extract (PAE) were respectively loaded on the shell and core layers of nanofibers by coaxial electrospinning technique. With the increase of Ss, the diameter of fiber decreased from 350 nm to 280 nm, the diameter of core layer increased from 120 nm to 140 nm, and the thickness of shell layer was reduced from 100 nm to 70 nm. The nanofiber membrane had good performance in mechanical properties and hydrophilicity with the addition of Ss. The results showed that the tensile strength of the nanofiber membrane could reach 4.31 MPa, the swelling rate could reach 150%, the water vapor transmission rate could reach 1834 g/(m2?24h) and the water contact angle could be reduced to 32.7 ?. The influence of drug burst release could be significantly inhibited by core-shell structure, and finally long-term drug release was achieved. The drug release reached 77% within 7 days. Furthermore, nanofiber membrane could effectively inhibit the growth of bacteria and promote cell proliferation. Compared with that of unloaded Ss, cell proliferation of nanofiber membrane loaded with 20% Ss was increased by 25%, which indicates that Ss and PAE play a synergistic role in wound healing.