纳米氧化铁及其水合物的生物学效应

采用水热法合成了纳米氧化铁及其水合物(羟基氧化铁),通过红外光谱、扫描电镜等进行分析,表明纳米氧化铁呈球形,而纳米羟基氧化铁呈棒状,纳米微粒的粒径在50～120 nm之间。将掺杂这两种纳米材料的氨基酸进行重结晶,光学显微镜观察发现其晶型发生了变化,红外光谱图谱出现了峰的移位和增减,说明纳米材料对氨基酸的晶体内部结构产生了影响。用MTT (噻唑蓝)细胞增值率实验评价材料的细胞毒性,两种纳米材料的细胞增值率约为70%～95%,细胞毒性评级1～2,表明其较低的细胞毒性。

Biological Effect of Iron Oxide and Its Hydrate Nanoparticles

Nanoparticles of iron oxide and its hydrate were synthesized with hydrothermal method and characterized by infrared, Scanning Electron Microscope (SEM) and soon. The dimensions of the nanoparticles are about 50~120 nm. The form of iron oxide nanoparticles appears to be globosity while its hydrate rod. Amino acids intermingling with the synthesized nanoparticles were crystallized to investigate the space effect of the nanoparticles. The crystalline forms of crystal are different from those of pure amino acid. The positions and width of the nanoparticles'peaks in the infrared spectrum change too. Microscope observation and infrared spectrum results indicate the nanoparticles have changed the internal structure of amino acids crystal. 3-(4, 5-dimethythiazol 2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay was used to determine their cytotoxicity. The Optical Density (OD) value was used to calculate Relative Generation Rate (RGR) of cells. The RGRs of nanoparticles of iron oxide and its hydrate are about 70%~95%, the grades of cytotoxicity are 1~2. This result indicates they just have low toxicity.