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The impact of fullerenol nanoparticles on the growth of toxigenic Aspergillus flavus and aflatoxins production in vitro and in corn flour |
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| Authors: | Jelena Živančev Sandra Bulut Sunčica Kocić-Tanackov Danica Jović Aleksandar Fišteš Igor Antić Aleksandar Djordjevic |
| Affiliation: | 1. Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, Serbia Contribution: Conceptualization, Methodology, Investigation, Formal analysis, Supervision, Funding acquisition, Visualization, Resources, Writing - original draft, Writing - review & editing;2. Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, Serbia Contribution: Methodology, Investigation, Formal analysis, Visualization, Writing - original draft, Writing - review & editing;3. Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, Serbia Contribution: Conceptualization, Methodology, Investigation, Formal analysis, Supervision, Visualization, Funding acquisition, Resources, Writing - original draft, Writing - review & editing;4. Faculty of Sciences, Department of Chemistry, Biochemistry, and Environmental Protection, University of Novi Sad, Novi Sad, Serbia;5. Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, Serbia Contribution: Conceptualization, Methodology, Investigation, Funding acquisition, Resources, Writing - original draft, Writing - review & editing, Supervision;6. Faculty of Sciences, Department of Chemistry, Biochemistry, and Environmental Protection, University of Novi Sad, Novi Sad, Serbia Contribution: Conceptualization, Methodology, Investigation, Supervision, Funding acquisition, Resources, Writing - original draft, Writing - review & editing |
| Abstract: | Antifungal and antimycotoxigenic activity of fullerenol nanoparticles (FNPs) were investigated on Aspergillus flavus growth isolated from a real food sample and aflatoxins (AFs) (AFB1 and AFB2) production. The final FNPs concentrations in in vitro and in commercial corn flour after the stationary incubation period of 7 and 14 days were in the range 0.16–80 µg/mL and 0.16–80 µg/g, respectively. Nanocharacterization of FNPs revealed an average size of 5–20 nm and a zeta potential of −35 mV. The highest degree of A. flavus mycelium growth inhibition (28%) after 7 days was observed for applied FNP concentration of 8.0 µg/mL, while after 14 days FNP concentration of 0.32 µg/mL led to the maximal inhibition of A. flavus mycelium growth (36%). Spearman's correlations analysis revealed a strong positive correlation between AFB1 and AFB2 concentrations in YES broth after 7 (R = 0.994, p < 0.05) and 14 days (R = 0.976), as well as between AFs concentrations and A. flavus mycelium mass after 7 (R = 0.786 for AFB1 and R = 0.766 for AFB2) and 14 days (R = 0.810 for AFB1 and R = 0.833 for AFB2). Paired samples t-test showed the existence of a statistically significant difference (p < 0.05) between the produced AFs concentrations after the incubation of 7 and 14 days. Regarding the artificially inoculated corn flour the lower applied FNP concentrations (0.16–0.8 µg/g) achieved a reduction of AFB1 up to 42% and 60% after 7 and 14 days, respectively. |
| Keywords: | aflatoxins food safety fullerenol nanoparticles liquid chromatograph nanomaterials |