Isolation,identification and activity of natural antioxidants from horehound (Marrubium vulgare L.) cultivated in Lithuania |
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| Authors: | Audrius Pukalskas Petras Rimantas Venskutonis Sofia Salido Pieter de Waard Teris A van Beek |
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| Affiliation: | 1. Department of Food Technology, Kaunas University of Technology, Radvil?n? pl. 19, LT-50254 Kaunas, Lithuania;2. Dpto. Quimica Inorganica y Organica, Facultad de Ciencias Experimentales, Universidad de Jaen, 23071 Jaen, Spain;3. Wageningen NMR Centre, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands;4. Laboratory of Organic Chemistry, Natural Products Chemistry and Microfluidics Group, Wageningen University, Dreijenplein 8, 6703 HB Wageningen, The Netherlands |
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| Abstract: | In an earlier screening of Lithuanian plants, horehound (Marrubium vulgare) showed good antioxidant activity and as this species is used in herbal teas and cough pastilles it was selected for further investigation. Some fractions of the aerial parts were strong scavengers of the model free radicals DPPH and ABTS+ . Activity in the β-carotene bleaching assay and the rapeseed oil oxidation assay was lower. Several active compounds were observed in the crude methanol–water extract, and in butanol and methyl tert-butyl ether fractions using HPLC with on-line radical scavenging detection. After multi-step fractionation of these fractions five compounds possessing radical scavenging activity were purified and their structures were elucidated by NMR and MS as 5,6-dihydroxy-7,4′-dimethoxyflavone (syn. ladanein), 7-O-β-glucopyranosyl luteolin, 7-O-β-glucuronyl luteolin, verbascoside and forsythoside B. Their activities were tested in off-line DPPH and ABTS+ free radical scavenging assays, and compared with the antioxidants rosmarinic acid and Trolox. |
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